Obtaining Hemocytes from the Hawaiian Bobtail Squid <em>Euprymna scolopes</em> and Observing their Adherence to Symbiotic and Non-Symbiotic Bacteria

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Obtaining Hemocytes from the Hawaiian Bobtail Squid Euprymna scolopes and Observing their Adherence to Symbiotic and Non-Symbiotic Bacteria

Studies concerning the role of the immune system in mediating molecular signaling between beneficial bacteria and their hosts have, in recent years, made significant contributions to our understanding of the co-evolution of eukaryotes with their microbiota. The symbiotic association between the Hawaiian bobtail squid, Euprymna scolopes and the bioluminescent bacterium Vibrio fischeri has been u...

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Hawaiian bobtail squid

What is a bobtail squid? Euprymna scolopes (Figure 1) is a small sepiolid squid, or bobtail squid, occurring in the marine coastal environment of the Hawaiian archipelago. Whereas most other squid are always out and about, the bobtail squid buries itself in the sand during its daylight quiescent period. Then, at night, individuals of this species emerge from the sand to forage on prey, such as ...

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The Role of Hemocytes in the Hawaiian Bobtail Squid, Euprymna scolopes: A Model Organism for Studying Beneficial Host–Microbe Interactions

Most, if not all, animals engage in associations with bacterial symbionts. Understanding the mechanisms by which host immune systems and beneficial bacteria communicate is a fundamental question in the fields of immunology and symbiosis. The Hawaiian bobtail squid (Euprymna scolopes) engages in two known symbioses; a binary relationship with the light organ symbiont Vibrio fischeri, and a bacte...

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Antibacterial Activities of Extracellular Metabolites of Symbiotic Bacteria, Xenorhabdus and Photorhabdus Isolated from Entomopathogenic Nematodes

Xenorhabdus and Photorhabdus are members of the family Enterobacteriaceae, and are engaged in a mutualistic association with the entomopathogenic nematodes, Steinernema and Heterorhabditis, respectively. In the present study, the organic fraction of ethyl acetate bacterial crude extracts of the symbiotic bacteria, Xenorhabdus stockiae and Photorhabdus luminescens isolated from entomopathogenic ...

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Entomopathogenic nematodes of the family Steinernematidae and their mutualistic bacteria (Xenorhabdus spp.) are lethal endoparasites of insects. We hypothesized that growth of the nematode's mutualistic bacteria in the insect host may contribute to the production of cues used by the infective juveniles (IJs) in responding to potential hosts for infection. Specifically, we tested if patterns of ...

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ژورنال

عنوان ژورنال: Journal of Visualized Experiments

سال: 2010

ISSN: 1940-087X

DOI: 10.3791/1714